Influence of Effciency of Different Oocyes Harvesting Methods on Oocyte Retrieval and Quality in Local Ewes (Ovis Aries)

Abstract

The aimed of present study to determine the influence  of different oocytes harvesting methods on oocyte retrieval and quality in Local ewes (Ovis aries). The genital organs of 225 (450 ovaries) for adult non - gravid ewes slaughtered at the slaughterhouses of Babylon provice  for duration from September 2020 to January 2021. The oocytes were obtained from ovaries by three methods include ovarian slicing, ovarian puncture and aspiration of follicle methods. The oocytes were graded as good, fair and poor oocytes on basis of cumulus cells, homogeneity of ooplasm and shape of oocyte.  The results showed a significant increase (p<0.05) in the  total number of oocytes and the average number of oocytes per each ovary by using ovarian slicing  method and amounted to 1032 and 6.88±0.06 respectively compared with ovarian puncture method and reached to 741 and 4.94±0.11 respectively and aspiration of follicle method and reached to 465 and 3.10±0.08 respectively. A significant increase (p<0.05) in the average number of oocytes per each ovary (3.12±0.13)  and the percentage of good oocytes 45.35% obtained by the ovarian slicing method observed compared to the ovarian puncture method (1.87±0.03 and 37.80 % respectively) and aspiration of follicle method (0.88±0.06 and 28.22% respectively) . According to this results can be concluded that the ovarian  slicing method was found the suitable and effective method for obtained a larger number and good quality of oocytes and least number of poor oocytes  compared with ovarian puncture method and aspiration of follicle method